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Dragomirka Jovic, Xue Liang, Hua Zeng, Lin Lin, Fengping Xu, Yonglun Luo
Single-cell RNA sequencing (scRNA-seq) technology has become the state-of-the-art approach for unravelling the heterogeneity and complexity of RNA transcripts within individual cells, as well as revealing the composition of different cell types and functions within highly organized tissues/organs/organisms. Since its first discovery in 2009, studies based on scRNA-seq provide massive information across different fields making exciting new discoveries in better understanding the composition and interaction of cells within humans, model animals and plants. In this review, we provide a concise overview about the scRNA-seq technology, experimental and computational procedures for transforming the biological and molecular processes into computational and statistical data. We also provide an explanation of the key technological steps in implementing the technology. We highlight a few examples on how scRNA-seq can provide unique information for better understanding health and diseases. One important application of the scRNA-seq technology is to build a better and high-resolution catalogue of cells in all living organism, commonly known as atlas, which is key resource to better understand and provide a solution in treating diseases. While great promises have been demonstrated with the technology in all areas, we further highlight a few remaining challenges to be overcome and its great potentials in transforming current protocols in disease diagnosis and treatment.
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Jovic, D., Liang, X., Zeng, H., Lin, L., Xu, F., & Luo, Y. (2022). Single‐cell RNA sequencing technologies and applications: A brief overview. Clinical and Translational Medicine, 12(3), e694. https://doi.org/10.1002/ctm2.694
Jovic, Dragomirka, et al. "Single‐cell RNA sequencing technologies and applications: A brief overview." Clinical and Translational Medicine, vol. 12, no. 3, 2022, pp. e694, https://doi.org/10.1002/ctm2.694.
Jovic, Dragomirka, Xue Liang, Hua Zeng, Lin Lin, Fengping Xu, and Yonglun Luo. "Single‐cell RNA sequencing technologies and applications: A brief overview." Clinical and Translational Medicine 12, no. 3 (2022): e694. https://doi.org/10.1002/ctm2.694.
Jovic D., Liang X., Zeng H. [и др.] Single‐cell RNA sequencing technologies and applications: A brief overview // Clinical and Translational Medicine. — 2022. — Vol. 12, № 3. — P. e694. — URL: https://doi.org/10.1002/ctm2.694
@article{jovic2022singlecell,
title = {{Single‐cell RNA sequencing technologies and applications: A brief overview}},
author = {Dragomirka Jovic and Xue Liang and Hua Zeng and Lin Lin and Fengping Xu and Yonglun Luo},
journal = {Clinical and Translational Medicine},
year = {2022},
volume = {12},
number = {3},
pages = {e694},
doi = {10.1002/ctm2.694},
url = {https://doi.org/10.1002/ctm2.694}
}TY - JOUR AU - Jovic, Dragomirka AU - Liang, Xue AU - Zeng, Hua AU - Lin, Lin AU - Xu, Fengping AU - Luo, Yonglun TI - Single‐cell RNA sequencing technologies and applications: A brief overview JO - Clinical and Translational Medicine VL - 12 IS - 3 SP - e694 PY - 2022 DA - 2022/03/01 DO - 10.1002/ctm2.694 UR - https://doi.org/10.1002/ctm2.694 ER -
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