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Sem Tamara, Maurits A. den Boer, Albert J. R. Heck
Native mass spectrometry (MS) involves the analysis and characterization of macromolecules, predominantly intact proteins and protein complexes, whereby as much as possible the native structural features of the analytes are retained. As such, native MS enables the study of secondary, tertiary, and even quaternary structure of proteins and other biomolecules. Native MS represents a relatively recent addition to the analytical toolbox of mass spectrometry and has over the past decade experienced immense growth, especially in enhancing sensitivity and resolving power but also in ease of use. With the advent of dedicated mass analyzers, sample preparation and separation approaches, targeted fragmentation techniques, and software solutions, the number of practitioners and novel applications has risen in both academia and industry. This review focuses on recent developments, particularly in high-resolution native MS, describing applications in the structural analysis of protein assemblies, proteoform profiling of─among others─biopharmaceuticals and plasma proteins, and quantitative and qualitative analysis of protein-ligand interactions, with the latter covering lipid, drug, and carbohydrate molecules, to name a few.
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Tamara, S., Boer, M. A. D., & Heck, A. J. R. (2021). High-Resolution Native Mass Spectrometry. Chemical Reviews, 122(8), 7269–7326. https://doi.org/10.1021/acs.chemrev.1c00212
Tamara, Sem, et al. "High-Resolution Native Mass Spectrometry." Chemical Reviews, vol. 122, no. 8, 2021, pp. 7269–7326, https://doi.org/10.1021/acs.chemrev.1c00212.
Tamara, Sem, Maurits A. den Boer, and Albert J. R. Heck. "High-Resolution Native Mass Spectrometry." Chemical Reviews 122, no. 8 (2021): 7269–7326. https://doi.org/10.1021/acs.chemrev.1c00212.
Tamara S., Boer M. A. D., Heck A. J. R. High-Resolution Native Mass Spectrometry // Chemical Reviews. — 2021. — Vol. 122, № 8. — P. 7269–7326. — URL: https://doi.org/10.1021/acs.chemrev.1c00212
@article{tamara2021highresolution,
title = {{High-Resolution Native Mass Spectrometry}},
author = {Sem Tamara and Maurits A. den Boer and Albert J. R. Heck},
journal = {Chemical Reviews},
year = {2021},
volume = {122},
number = {8},
pages = {7269--7326},
doi = {10.1021/acs.chemrev.1c00212},
url = {https://doi.org/10.1021/acs.chemrev.1c00212}
}TY - JOUR AU - Tamara, Sem AU - Boer, Maurits A. den AU - Heck, Albert J. R. TI - High-Resolution Native Mass Spectrometry JO - Chemical Reviews VL - 122 IS - 8 SP - 7269 EP - 7326 PY - 2021 DA - 2021/08/20 DO - 10.1021/acs.chemrev.1c00212 UR - https://doi.org/10.1021/acs.chemrev.1c00212 ER -
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