One connector, four scholarly indexes, real DOIs and legal free PDFs. Works in Claude and ChatGPT.
Paste it into Claude or ChatGPT as a custom connector. 3-day free trial.
Tingting Zhu, Le Wang, Hélène Rimbert, J. Rodriguez, Karin R. Deal, Romain De Oliveira, Frédéric Choulet, Gabriel Keeble‐Gagnère, Josquin F. G. Tibbits, Jane Rogers and 6 more
Until recently, achieving a reference-quality genome sequence for bread wheat was long thought beyond the limits of genome sequencing and assembly technology, primarily due to the large genome size and > 80% repetitive sequence content. The release of the chromosome scale 14.5-Gb IWGSC RefSeq v1.0 genome sequence of bread wheat cv. Chinese Spring (CS) was, therefore, a milestone. Here, we used a direct label and stain (DLS) optical map of the CS genome together with a prior nick, label, repair and stain (NLRS) optical map, and sequence contigs assembled with Pacific Biosciences long reads, to refine the v1.0 assembly. Inconsistencies between the sequence and maps were reconciled and gaps were closed. Gap filling and anchoring of 279 unplaced scaffolds increased the total length of pseudomolecules by 168 Mb (excluding Ns). Positions and orientations were corrected for 233 and 354 scaffolds, respectively, representing 10% of the genome sequence. The accuracy of the remaining 90% of the assembly was validated. As a result of the increased contiguity, the numbers of transposable elements (TEs) and intact TEs have increased in IWGSC RefSeq v2.1 compared with v1.0. In total, 98% of the gene models identified in v1.0 were mapped onto this new assembly through development of a dedicated approach implemented in the MAGAAT pipeline. The numbers of high-confidence genes on pseudomolecules have increased from 105 319 to 105 534. The reconciled assembly enhances the utility of the sequence for genetic mapping, comparative genomics, gene annotation and isolation, and more general studies on the biology of wheat.
Formatted from the source metadata, not typed by hand. For a whole reference list, use the citation generator or ask your assistant through Find Research.
Zhu, T., Wang, L., Rimbert, H., Rodriguez, J., Deal, K. R., Oliveira, R. D., Choulet, F., Keeble‐Gagnère, G., Tibbits, J. F. G., Rogers, J., Eversole, K., Appels, R., Gu, Y., Mascher, M., Dvořák, J., & Luo, M. (2021). Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly. The Plant Journal, 107(1), 303–314. https://doi.org/10.1111/tpj.15289
Zhu, Tingting, et al. "Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly." The Plant Journal, vol. 107, no. 1, 2021, pp. 303–314, https://doi.org/10.1111/tpj.15289.
Zhu, Tingting, Le Wang, Hélène Rimbert, J. Rodriguez, Karin R. Deal, Romain De Oliveira, Frédéric Choulet, et al. "Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly." The Plant Journal 107, no. 1 (2021): 303–314. https://doi.org/10.1111/tpj.15289.
Zhu T., Wang L., Rimbert H. [и др.] Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly // The Plant Journal. — 2021. — Vol. 107, № 1. — P. 303–314. — URL: https://doi.org/10.1111/tpj.15289
@article{zhu2021optical,
title = {{Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly}},
author = {Tingting Zhu and Le Wang and Hélène Rimbert and J. Rodriguez and Karin R. Deal and Romain De Oliveira and Frédéric Choulet and Gabriel Keeble‐Gagnère and Josquin F. G. Tibbits and Jane Rogers and Kellye Eversole and R. Appels and Yong Gu and Martin Mascher and Jan Dvořák and Ming‐Cheng Luo},
journal = {The Plant Journal},
year = {2021},
volume = {107},
number = {1},
pages = {303--314},
doi = {10.1111/tpj.15289},
url = {https://doi.org/10.1111/tpj.15289}
}TY - JOUR AU - Zhu, Tingting AU - Wang, Le AU - Rimbert, Hélène AU - Rodriguez, J. AU - Deal, Karin R. AU - Oliveira, Romain De AU - Choulet, Frédéric AU - Keeble‐Gagnère, Gabriel AU - Tibbits, Josquin F. G. AU - Rogers, Jane AU - Eversole, Kellye AU - Appels, R. AU - Gu, Yong AU - Mascher, Martin AU - Dvořák, Jan AU - Luo, Ming‐Cheng TI - Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly JO - The Plant Journal VL - 107 IS - 1 SP - 303 EP - 314 PY - 2021 DA - 2021/04/24 DO - 10.1111/tpj.15289 UR - https://doi.org/10.1111/tpj.15289 ER -
Add Find Research to Claude or ChatGPT and ask in plain language. The assistant reads this record, finds the papers around it and cites only what it actually found:
Setup takes about a minute: add the connector, then start the free trial.
Record from OpenAlex; open-access location from Unpaywall and the indexes. Find Research links only to legal copies.