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Ingvill Pedersen Sæbø, Magnar Bjørås, Henrik Franzyk, Emily Helgesen, James Alexander Booth
In vitro determination of hemolytic properties is a common and important method for preliminary evaluation of cytotoxicity of chemicals, drugs, or any blood-contacting medical device or material. The method itself is relatively straightforward, however, protocols used in the literature vary substantially. This leads to significant difficulties both in interpreting and in comparing the obtained values. Here, we examine how the different variables used under different experimental setups may affect the outcome of this assay. We find that certain key parameters affect the hemolysis measurements in a critical manner. The hemolytic effect of compounds tested here varied up to fourfold depending on the species of the blood source. The use of different types of detergents used for generating positive control samples (i.e., 100% hemolysis) produced up to 2.7-fold differences in the calculated hemolysis ratios. Furthermore, we find an expected, but substantial, increase in the number of hemolyzed erythrocytes with increasing erythrocyte concentration and with prolonged incubation time, which in turn affects the calculated hemolysis ratios. Based on our findings we propose an optimized protocol in an attempt to standardize future hemolysis studies.
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Sæbø, I. P., Bjørås, M., Franzyk, H., Helgesen, E., & Booth, J. A. (2023). Optimization of the Hemolysis Assay for the Assessment of Cytotoxicity. International Journal of Molecular Sciences, 24(3), 2914. https://doi.org/10.3390/ijms24032914
Sæbø, Ingvill Pedersen, et al. "Optimization of the Hemolysis Assay for the Assessment of Cytotoxicity." International Journal of Molecular Sciences, vol. 24, no. 3, 2023, pp. 2914, https://doi.org/10.3390/ijms24032914.
Sæbø, Ingvill Pedersen, Magnar Bjørås, Henrik Franzyk, Emily Helgesen, and James Alexander Booth. "Optimization of the Hemolysis Assay for the Assessment of Cytotoxicity." International Journal of Molecular Sciences 24, no. 3 (2023): 2914. https://doi.org/10.3390/ijms24032914.
Sæbø I. P., Bjørås M., Franzyk H. [и др.] Optimization of the Hemolysis Assay for the Assessment of Cytotoxicity // International Journal of Molecular Sciences. — 2023. — Vol. 24, № 3. — P. 2914. — URL: https://doi.org/10.3390/ijms24032914
@article{sb2023optimization,
title = {{Optimization of the Hemolysis Assay for the Assessment of Cytotoxicity}},
author = {Ingvill Pedersen Sæbø and Magnar Bjørås and Henrik Franzyk and Emily Helgesen and James Alexander Booth},
journal = {International Journal of Molecular Sciences},
year = {2023},
volume = {24},
number = {3},
pages = {2914},
doi = {10.3390/ijms24032914},
url = {https://doi.org/10.3390/ijms24032914}
}TY - JOUR AU - Sæbø, Ingvill Pedersen AU - Bjørås, Magnar AU - Franzyk, Henrik AU - Helgesen, Emily AU - Booth, James Alexander TI - Optimization of the Hemolysis Assay for the Assessment of Cytotoxicity JO - International Journal of Molecular Sciences VL - 24 IS - 3 SP - 2914 PY - 2023 DA - 2023/02/02 DO - 10.3390/ijms24032914 UR - https://doi.org/10.3390/ijms24032914 ER -
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